rat cd25 fitc Search Results


anti cd25  (Cedarlane)
93
Cedarlane anti cd25
Vβ10 expression by CD8+ <t>CD25+</t> activated T cells from the draining lymph nodes of HSV-1-infected mice determined directly ex vivo or after 3 days of culture without exogenous antigen. Three mice were infected in the hind footpads with 4 × 105 PFU of HSV-1, and 5 days later the cells from the draining popliteal lymph nodes were collected, viable cell counts were performed, and 1 × 106 to 2 × 106 cells were placed into culture without exogenous antigen. Another 106 cells were triple stained with Vβ10, CD25, and CD8 antibodies prior to analysis by flow cytometry. After 3 days the cultured cells were similarly analyzed by flow cytometry. Dead cells were excluded by using propidium iodide staining. The dot plots represent CD8 versus CD25 staining for the popliteal lymph node cells ex vivo, and after 3 days in culture without exogenous antigen (in vitro). The histogram shows the Vβ10 receptor expression of the CD8+ CD25+ T cell subsets. The percentage of CD8+ CD25+ cells in the lymph nodes, and the percentage of Vβ10+ T cells among the CD8+ CD25+ T cell subsets are shown as a mean and standard deviation for the three mice analyzed.
Anti Cd25, supplied by Cedarlane, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rat+cd25+fitc/pmc00111723-51-20-22?v=Cedarlane
Average 93 stars, based on 1 article reviews
anti cd25 - by Bioz Stars, 2026-08
93/100 stars
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anti human p75 ntr antibodies  (Cedarlane)
90
Cedarlane anti human p75 ntr antibodies
Vβ10 expression by CD8+ <t>CD25+</t> activated T cells from the draining lymph nodes of HSV-1-infected mice determined directly ex vivo or after 3 days of culture without exogenous antigen. Three mice were infected in the hind footpads with 4 × 105 PFU of HSV-1, and 5 days later the cells from the draining popliteal lymph nodes were collected, viable cell counts were performed, and 1 × 106 to 2 × 106 cells were placed into culture without exogenous antigen. Another 106 cells were triple stained with Vβ10, CD25, and CD8 antibodies prior to analysis by flow cytometry. After 3 days the cultured cells were similarly analyzed by flow cytometry. Dead cells were excluded by using propidium iodide staining. The dot plots represent CD8 versus CD25 staining for the popliteal lymph node cells ex vivo, and after 3 days in culture without exogenous antigen (in vitro). The histogram shows the Vβ10 receptor expression of the CD8+ CD25+ T cell subsets. The percentage of CD8+ CD25+ cells in the lymph nodes, and the percentage of Vβ10+ T cells among the CD8+ CD25+ T cell subsets are shown as a mean and standard deviation for the three mice analyzed.
Anti Human P75 Ntr Antibodies, supplied by Cedarlane, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rat+cd25+fitc/10__1172_slash_jci119772-51-103-110?v=Cedarlane
Average 90 stars, based on 1 article reviews
anti human p75 ntr antibodies - by Bioz Stars, 2026-08
90/100 stars
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fitc conjugated anti cd25  (Cedarlane)
85
Cedarlane fitc conjugated anti cd25
Vβ10 expression by CD8+ <t>CD25+</t> activated T cells from the draining lymph nodes of HSV-1-infected mice determined directly ex vivo or after 3 days of culture without exogenous antigen. Three mice were infected in the hind footpads with 4 × 105 PFU of HSV-1, and 5 days later the cells from the draining popliteal lymph nodes were collected, viable cell counts were performed, and 1 × 106 to 2 × 106 cells were placed into culture without exogenous antigen. Another 106 cells were triple stained with Vβ10, CD25, and CD8 antibodies prior to analysis by flow cytometry. After 3 days the cultured cells were similarly analyzed by flow cytometry. Dead cells were excluded by using propidium iodide staining. The dot plots represent CD8 versus CD25 staining for the popliteal lymph node cells ex vivo, and after 3 days in culture without exogenous antigen (in vitro). The histogram shows the Vβ10 receptor expression of the CD8+ CD25+ T cell subsets. The percentage of CD8+ CD25+ cells in the lymph nodes, and the percentage of Vβ10+ T cells among the CD8+ CD25+ T cell subsets are shown as a mean and standard deviation for the three mice analyzed.
Fitc Conjugated Anti Cd25, supplied by Cedarlane, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rat+cd25+fitc/pm09218758-46-43-48?v=Cedarlane
Average 85 stars, based on 1 article reviews
fitc conjugated anti cd25 - by Bioz Stars, 2026-08
85/100 stars
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anti-rat cd4 fitc/ cd25 rpe dual color reagent  (MorphoSys ag)
90
MorphoSys ag anti-rat cd4 fitc/ cd25 rpe dual color reagent
Vβ10 expression by CD8+ <t>CD25+</t> activated T cells from the draining lymph nodes of HSV-1-infected mice determined directly ex vivo or after 3 days of culture without exogenous antigen. Three mice were infected in the hind footpads with 4 × 105 PFU of HSV-1, and 5 days later the cells from the draining popliteal lymph nodes were collected, viable cell counts were performed, and 1 × 106 to 2 × 106 cells were placed into culture without exogenous antigen. Another 106 cells were triple stained with Vβ10, CD25, and CD8 antibodies prior to analysis by flow cytometry. After 3 days the cultured cells were similarly analyzed by flow cytometry. Dead cells were excluded by using propidium iodide staining. The dot plots represent CD8 versus CD25 staining for the popliteal lymph node cells ex vivo, and after 3 days in culture without exogenous antigen (in vitro). The histogram shows the Vβ10 receptor expression of the CD8+ CD25+ T cell subsets. The percentage of CD8+ CD25+ cells in the lymph nodes, and the percentage of Vβ10+ T cells among the CD8+ CD25+ T cell subsets are shown as a mean and standard deviation for the three mice analyzed.
Anti Rat Cd4 Fitc/ Cd25 Rpe Dual Color Reagent, supplied by MorphoSys ag, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rat+cd25+fitc/pm20064086-95-21-30?v=MorphoSys+ag
Average 90 stars, based on 1 article reviews
anti-rat cd4 fitc/ cd25 rpe dual color reagent - by Bioz Stars, 2026-08
90/100 stars
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cd25 rat mab (fitc)  (Aladdin Scientific Corporation)
N/A
Application:Flow: 5μL/testFor flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume.
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fitc anti-rat cd25  (BioLegend)
N/A
FITC anti-rat CD25 [OX-39]; Isotype: Mouse IgG1, κ; Reactivity: Rat; Apps: FC; Size: 100 μg
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fitc rat anti mouse cd25  (BD Biosciences)
N/A
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fitc mouse anti rat cd25  (BD Biosciences)
N/A
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mouse anti rat cd4:fitc/cd25:rpe dual color reagent (100tests)  (Aviva Systems)
N/A
MOUSE ANTI RAT CD4:FITC/CD25:RPE; MOUSE ANTI RAT CD4:FITC/CD25:RPE_x000D_
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Image Search Results


Vβ10 expression by CD8+ CD25+ activated T cells from the draining lymph nodes of HSV-1-infected mice determined directly ex vivo or after 3 days of culture without exogenous antigen. Three mice were infected in the hind footpads with 4 × 105 PFU of HSV-1, and 5 days later the cells from the draining popliteal lymph nodes were collected, viable cell counts were performed, and 1 × 106 to 2 × 106 cells were placed into culture without exogenous antigen. Another 106 cells were triple stained with Vβ10, CD25, and CD8 antibodies prior to analysis by flow cytometry. After 3 days the cultured cells were similarly analyzed by flow cytometry. Dead cells were excluded by using propidium iodide staining. The dot plots represent CD8 versus CD25 staining for the popliteal lymph node cells ex vivo, and after 3 days in culture without exogenous antigen (in vitro). The histogram shows the Vβ10 receptor expression of the CD8+ CD25+ T cell subsets. The percentage of CD8+ CD25+ cells in the lymph nodes, and the percentage of Vβ10+ T cells among the CD8+ CD25+ T cell subsets are shown as a mean and standard deviation for the three mice analyzed.

Journal:

Article Title: Herpes Simplex Virus Type 1-Specific Cytotoxic T-Lymphocyte Arming Occurs within Lymph Nodes Draining the Site of Cutaneous Infection

doi:

Figure Lengend Snippet: Vβ10 expression by CD8+ CD25+ activated T cells from the draining lymph nodes of HSV-1-infected mice determined directly ex vivo or after 3 days of culture without exogenous antigen. Three mice were infected in the hind footpads with 4 × 105 PFU of HSV-1, and 5 days later the cells from the draining popliteal lymph nodes were collected, viable cell counts were performed, and 1 × 106 to 2 × 106 cells were placed into culture without exogenous antigen. Another 106 cells were triple stained with Vβ10, CD25, and CD8 antibodies prior to analysis by flow cytometry. After 3 days the cultured cells were similarly analyzed by flow cytometry. Dead cells were excluded by using propidium iodide staining. The dot plots represent CD8 versus CD25 staining for the popliteal lymph node cells ex vivo, and after 3 days in culture without exogenous antigen (in vitro). The histogram shows the Vβ10 receptor expression of the CD8+ CD25+ T cell subsets. The percentage of CD8+ CD25+ cells in the lymph nodes, and the percentage of Vβ10+ T cells among the CD8+ CD25+ T cell subsets are shown as a mean and standard deviation for the three mice analyzed.

Article Snippet: Lymph node cells cultured for 3 days without exogenous antigen were also stained with allophycocyanin-labeled anti-CD8 (53-6.7; Pharmingen) and FITC-labeled anti-CD25 (PC61.5.3; Cedarlane Laboratories, Ltd., Hornby, Ontario, Canada).

Techniques: Expressing, Infection, Ex Vivo, Staining, Flow Cytometry, Cell Culture, In Vitro, Standard Deviation

The CD8+ CD25+ T cells from the draining lymph nodes of HSV-1-infected mice analyzed after 3 days of culture without exogenous antigen are predominantly gB specific. A mouse was infected in the hind footpads with 4 × 105 PFU of HSV-1, and 5 days later the cells from the draining popliteal lymph nodes were collected and placed into culture without exogenous antigen. After 3 days, the cultured cells and lymph node cells isolated from a naive mouse were triple stained with CD25 and CD8 antibodies, and the H-2Kb/gB tetramer and analyzed by flow cytometry. Dead cells were excluded by using propidium iodide staining. The dot plot represents CD8 versus CD25 staining for the cultured popliteal lymph node cells. The histograms show the level of H-2Kb/gB tetramer staining of the CD8+ CD25hi and CD8+ CD25lo T cell subsets from the cultured (shaded) and naive (unshaded) lymph node cell populations.

Journal:

Article Title: Herpes Simplex Virus Type 1-Specific Cytotoxic T-Lymphocyte Arming Occurs within Lymph Nodes Draining the Site of Cutaneous Infection

doi:

Figure Lengend Snippet: The CD8+ CD25+ T cells from the draining lymph nodes of HSV-1-infected mice analyzed after 3 days of culture without exogenous antigen are predominantly gB specific. A mouse was infected in the hind footpads with 4 × 105 PFU of HSV-1, and 5 days later the cells from the draining popliteal lymph nodes were collected and placed into culture without exogenous antigen. After 3 days, the cultured cells and lymph node cells isolated from a naive mouse were triple stained with CD25 and CD8 antibodies, and the H-2Kb/gB tetramer and analyzed by flow cytometry. Dead cells were excluded by using propidium iodide staining. The dot plot represents CD8 versus CD25 staining for the cultured popliteal lymph node cells. The histograms show the level of H-2Kb/gB tetramer staining of the CD8+ CD25hi and CD8+ CD25lo T cell subsets from the cultured (shaded) and naive (unshaded) lymph node cell populations.

Article Snippet: Lymph node cells cultured for 3 days without exogenous antigen were also stained with allophycocyanin-labeled anti-CD8 (53-6.7; Pharmingen) and FITC-labeled anti-CD25 (PC61.5.3; Cedarlane Laboratories, Ltd., Hornby, Ontario, Canada).

Techniques: Infection, Cell Culture, Isolation, Staining, Flow Cytometry